ssr_primer
Primer Details for TRINITY_DN76268_c3_g2_i1 |
|---|
| (T)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCAGTAGGGCGGGTCATC | CCATGGTAACCCCTTCCACC | Start: 1752 |
| Tm | 59.966 | 60.034 | End: 1876 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | ATGTTGCCTTCCTCTCCAGC | GCAACCTGGTTCCCGTAGAA | Start: 886 |
| Tm | 60.035 | 59.964 | End: 985 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | ATGTTGACGATGCCGGGAAT | CCATGGTAACCCCTTCCACC | Start: 1653 |
| Tm | 60.107 | 60.034 | End: 1876 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | ATGTTGACGATGCCGGGAAT | GATGACCCGCCCTACTGATG | Start: 1653 |
| Tm | 60.107 | 59.966 | End: 1771 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | GGACCATTGGGAGGAGTTCG | GATGACCCGCCCTACTGATG | Start: 1593 |
| Tm | 60.108 | 59.966 | End: 1771 |
| Size | 20 | 20 | 179 |
(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATCAGTAGGGCGGGTCATC | CCATGGTAACCCCTTCCACC | Start: 1752 |
| Tm | 59.966 | 60.034 | End: 1876 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | ATGTTGCCTTCCTCTCCAGC | GCAACCTGGTTCCCGTAGAA | Start: 886 |
| Tm | 60.035 | 59.964 | End: 985 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | ATGTTGACGATGCCGGGAAT | CCATGGTAACCCCTTCCACC | Start: 1653 |
| Tm | 60.107 | 60.034 | End: 1876 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | ATGTTGACGATGCCGGGAAT | GATGACCCGCCCTACTGATG | Start: 1653 |
| Tm | 60.107 | 59.966 | End: 1771 |
| Size | 20 | 20 | 119 |
| Primer 5 details |
|---|
| Sequence | GGACCATTGGGAGGAGTTCG | GATGACCCGCCCTACTGATG | Start: 1593 |
| Tm | 60.108 | 59.966 | End: 1771 |
| Size | 20 | 20 | 179 |
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