Primer Details for TRINITY_DN76247_c3_g4_i1 | |||
|---|---|---|---|
| (A)39: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTGATACAAGAGAAAGCACCT | CATTTCTCCCTAAGTTGCGCC | Start: 144 |
| Tm | 59.716 | 59.598 | End: 243 |
| Size | 24 | 21 | 100 |
| Primer 2 details | |||
| Sequence | ACGAGAAAAGGCTACAAAATCTCT | TAAGTTGCGCCGTTCCAGTA | Start: 80 |
| Tm | 58.448 | 59.682 | End: 233 |
| Size | 24 | 20 | 154 |
| Primer 3 details | |||
| Sequence | ACAAACGAGAAAAGGCTACAAAA | TAAGTTGCGCCGTTCCAGTA | Start: 76 |
| Tm | 57.43 | 59.682 | End: 233 |
| Size | 23 | 20 | 158 |
| Primer 4 details | |||
| Sequence | TGTCGTCTTTCAGTGATACAAGAGA | TTTCTCCCTAAGTTGCGCCG | Start: 135 |
| Tm | 59.759 | 60.672 | End: 241 |
| Size | 25 | 20 | 107 |
| Primer 5 details | |||
| Sequence | CGAGAAAAGGCTACAAAATCTCTCA | TAAGTTGCGCCGTTCCAGTA | Start: 81 |
| Tm | 59.36 | 59.682 | End: 233 |
| Size | 25 | 20 | 153 |
