ssr_primer
Primer Details for TRINITY_DN76238_c7_g3_i5 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCGAAGGAATTCAGCGCA | GCCAAGTTCGTGGTTTGACC | Start: 764 |
| Tm | 60.037 | 59.97 | End: 1020 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
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| Sequence | CGGTGAGGGAGGAGAGAGAA | TGCGCTGAATTCCTTCGACT | Start: 525 |
| Tm | 60.034 | 60.037 | End: 783 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
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| Sequence | GGTCAAACCACGAACTTGGC | AACCAGTATCGCGGATGCAA | Start: 1001 |
| Tm | 59.97 | 60.108 | End: 1174 |
| Size | 20 | 20 | 174 |
| Primer 4 details |
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| Sequence | GGTCAAACCACGAACTTGGC | AAACCAGTATCGCGGATGCA | Start: 1001 |
| Tm | 59.97 | 60.108 | End: 1175 |
| Size | 20 | 20 | 175 |
| Primer 5 details |
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| Sequence | GGTCAAACCACGAACTTGGC | ATCGCGGATGCAAGTCGTAA | Start: 1001 |
| Tm | 59.97 | 60.179 | End: 1167 |
| Size | 20 | 20 | 167 |
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