ssr_primer
Primer Details for TRINITY_DN76155_c7_g2_i1 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGGCATGAGCGTTCCTTT | CCGCACGTGGTGTTCAAAAT | Start: 440 |
| Tm | 59.963 | 59.97 | End: 653 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | GCCTAAACGCTTGCTGATCG | TGTGCGATTTTTCTGCCACG | Start: 1100 |
| Tm | 59.973 | 60.041 | End: 1359 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | TCTGGCATGAGCGTTCCTTT | ATATTGACCGCACGTGGTGT | Start: 440 |
| Tm | 59.963 | 60.037 | End: 660 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
|---|
| Sequence | GCATGCTATTGGTGCGGATG | GACGTTTGCAGCACAGAAGG | Start: 1635 |
| Tm | 60.04 | 60.041 | End: 1847 |
| Size | 20 | 20 | 213 |
| Primer 5 details |
|---|
| Sequence | GCATGCTATTGGTGCGGATG | GGACGTTTGCAGCACAGAAG | Start: 1635 |
| Tm | 60.04 | 60.041 | End: 1848 |
| Size | 20 | 20 | 214 |
(AAGA)5a(AGT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGGCATGAGCGTTCCTTT | CCGCACGTGGTGTTCAAAAT | Start: 440 |
| Tm | 59.963 | 59.97 | End: 653 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
|---|
| Sequence | GCCTAAACGCTTGCTGATCG | TGTGCGATTTTTCTGCCACG | Start: 1100 |
| Tm | 59.973 | 60.041 | End: 1359 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | TCTGGCATGAGCGTTCCTTT | ATATTGACCGCACGTGGTGT | Start: 440 |
| Tm | 59.963 | 60.037 | End: 660 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
|---|
| Sequence | GCATGCTATTGGTGCGGATG | GACGTTTGCAGCACAGAAGG | Start: 1635 |
| Tm | 60.04 | 60.041 | End: 1847 |
| Size | 20 | 20 | 213 |
| Primer 5 details |
|---|
| Sequence | GCATGCTATTGGTGCGGATG | GGACGTTTGCAGCACAGAAG | Start: 1635 |
| Tm | 60.04 | 60.041 | End: 1848 |
| Size | 20 | 20 | 214 |
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