ssr_primer
Primer Details for TRINITY_DN76112_c9_g1_i1 |
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| (G)13cgggt(G)11t(G)10tggaacccccgc(G)14t(G)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGTCCCCTCCGGAAGTTTT | TTTTCTCAGTGGTCGGCGAC | Start: 80 |
| Tm | 59.309 | 60.599 | End: 303 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
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| Sequence | GAGTCCCCTCCGGAAGTTTT | GCCACAGGCCTCTAGTTGTA | Start: 80 |
| Tm | 59.309 | 59.097 | End: 274 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
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| Sequence | AGTCCCCTCCGGAAGTTTTTC | TTTTCTCAGTGGTCGGCGAC | Start: 81 |
| Tm | 59.927 | 60.599 | End: 303 |
| Size | 21 | 20 | 223 |
| Primer 4 details |
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| Sequence | GAGTCCCCTCCGGAAGTTTTT | TTTTCTCAGTGGTCGGCGAC | Start: 80 |
| Tm | 59.927 | 60.599 | End: 303 |
| Size | 21 | 20 | 224 |
| Primer 5 details |
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| Sequence | GAGTCCCCTCCGGAAGTTTT | TTTCTCAGTGGTCGGCGAC | Start: 80 |
| Tm | 59.309 | 60.007 | End: 302 |
| Size | 20 | 19 | 223 |
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