ssr_primer
Primer Details for TRINITY_DN76093_c1_g1_i5 |
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| (T)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTGCTGCATCAAAAACGCC | GCGCGGATGGTCGAATAGTA | Start: 614 |
| Tm | 60.039 | 60.04 | End: 728 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
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| Sequence | GATGGACGCTTTGTGTGAGC | ATTGCACTTGTACCCGCAGA | Start: 439 |
| Tm | 59.833 | 59.964 | End: 704 |
| Size | 20 | 20 | 266 |
| Primer 3 details |
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| Sequence | GATGGACGCTTTGTGTGAGC | GGCGTTTTTGATGCAGCAGA | Start: 439 |
| Tm | 59.833 | 60.039 | End: 633 |
| Size | 20 | 20 | 195 |
| Primer 4 details |
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| Sequence | TCTGCTGCATCAAAAACGCC | TCGAGTGATACTGTGCCACG | Start: 614 |
| Tm | 60.039 | 59.83 | End: 747 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
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| Sequence | GATGGACGCTTTGTGTGAGC | CATTGCACTTGTACCCGCAG | Start: 439 |
| Tm | 59.833 | 59.831 | End: 705 |
| Size | 20 | 20 | 267 |
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