ssr_primer
Primer Details for TRINITY_DN76088_c6_g3_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGGCGCATTTGTTGGACC | TGCTGTGAACTGTGAACCGT | Start: 534 |
| Tm | 59.967 | 60.108 | End: 692 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
|---|
| Sequence | TCCTGCATTTCATGGCACCA | GGTCCAACAAATGCGCCTTT | Start: 287 |
| Tm | 60.252 | 59.967 | End: 553 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
|---|
| Sequence | AAAGGCGCATTTGTTGGACC | GTGCTGTGAACTGTGAACCG | Start: 534 |
| Tm | 59.967 | 59.695 | End: 693 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | GGCGCATTTGTTGGACCAAA | TGCTGTGAACTGTGAACCGT | Start: 537 |
| Tm | 60.25 | 60.108 | End: 692 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | GAGGGGCAAAACGCATGAAA | TGCTGTGAACTGTGAACCGT | Start: 568 |
| Tm | 59.683 | 60.108 | End: 692 |
| Size | 20 | 20 | 125 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAGGCGCATTTGTTGGACC | TGCTGTGAACTGTGAACCGT | Start: 534 |
| Tm | 59.967 | 60.108 | End: 692 |
| Size | 20 | 20 | 159 |
| Primer 2 details |
|---|
| Sequence | TCCTGCATTTCATGGCACCA | GGTCCAACAAATGCGCCTTT | Start: 287 |
| Tm | 60.252 | 59.967 | End: 553 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
|---|
| Sequence | AAAGGCGCATTTGTTGGACC | GTGCTGTGAACTGTGAACCG | Start: 534 |
| Tm | 59.967 | 59.695 | End: 693 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | GGCGCATTTGTTGGACCAAA | TGCTGTGAACTGTGAACCGT | Start: 537 |
| Tm | 60.25 | 60.108 | End: 692 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | GAGGGGCAAAACGCATGAAA | TGCTGTGAACTGTGAACCGT | Start: 568 |
| Tm | 59.683 | 60.108 | End: 692 |
| Size | 20 | 20 | 125 |
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