ssr_primer
Primer Details for TRINITY_DN76075_c1_g1_i4 |
|---|
| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGCGTACACCGTACCTAC | TTGGCTCTCCTTTGTCCGAC | Start: 0 |
| Tm | 59.973 | 59.965 | End: 185 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | CCCCAAACAGGCAGCATCTA | CGTCCTCTTCCGATTCCTCG | Start: 1018 |
| Tm | 60.034 | 59.97 | End: 1151 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | GTCGGACAAAGGAGAGCCAA | TGCAGGGGCTTTAATGGGAG | Start: 166 |
| Tm | 59.965 | 60.033 | End: 349 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | TAGTGTACGCCTCAGCCTCT | TGGAAATTGAGATGCGCCCT | Start: 2485 |
| Tm | 60.034 | 60.034 | End: 2650 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | TGTTCGAATGCCTTCCGTGA | TTGGCTCTCCTTTGTCCGAC | Start: 61 |
| Tm | 59.966 | 59.965 | End: 185 |
| Size | 20 | 20 | 125 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCGCGTACACCGTACCTAC | TTGGCTCTCCTTTGTCCGAC | Start: 0 |
| Tm | 59.973 | 59.965 | End: 185 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
|---|
| Sequence | CCCCAAACAGGCAGCATCTA | CGTCCTCTTCCGATTCCTCG | Start: 1018 |
| Tm | 60.034 | 59.97 | End: 1151 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | GTCGGACAAAGGAGAGCCAA | TGCAGGGGCTTTAATGGGAG | Start: 166 |
| Tm | 59.965 | 60.033 | End: 349 |
| Size | 20 | 20 | 184 |
| Primer 4 details |
|---|
| Sequence | TAGTGTACGCCTCAGCCTCT | TGGAAATTGAGATGCGCCCT | Start: 2485 |
| Tm | 60.034 | 60.034 | End: 2650 |
| Size | 20 | 20 | 166 |
| Primer 5 details |
|---|
| Sequence | TGTTCGAATGCCTTCCGTGA | TTGGCTCTCCTTTGTCCGAC | Start: 61 |
| Tm | 59.966 | 59.965 | End: 185 |
| Size | 20 | 20 | 125 |
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