ssr_primer
Primer Details for TRINITY_DN76056_c5_g5_i1 |
|---|
| (C)19: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCAACCTGACCGCTCCG | TTGGCAATAAGGGAGAGCGA | Start: 43 |
| Tm | 60.433 | 59.091 | End: 175 |
| Size | 18 | 20 | 133 |
| Primer 2 details |
|---|
| Sequence | CTCCGCGATTGAACCCCC | TTGGCAATAAGGGAGAGCGA | Start: 56 |
| Tm | 60.514 | 59.091 | End: 175 |
| Size | 18 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | GTCAACCTGACCGCTCCG | GGCAATAAGGGAGAGCGATCA | Start: 43 |
| Tm | 60.433 | 59.929 | End: 173 |
| Size | 18 | 21 | 131 |
| Primer 4 details |
|---|
| Sequence | GTCAACCTGACCGCTCCG | TGGCAATAAGGGAGAGCGATC | Start: 43 |
| Tm | 60.433 | 59.929 | End: 174 |
| Size | 18 | 21 | 132 |
| Primer 5 details |
|---|
| Sequence | GTCAACCTGACCGCTCCG | TGGCAATAAGGGAGAGCGAT | Start: 43 |
| Tm | 60.433 | 58.868 | End: 174 |
| Size | 18 | 20 | 132 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCAACCTGACCGCTCCG | TTGGCAATAAGGGAGAGCGA | Start: 43 |
| Tm | 60.433 | 59.091 | End: 175 |
| Size | 18 | 20 | 133 |
| Primer 2 details |
|---|
| Sequence | CTCCGCGATTGAACCCCC | TTGGCAATAAGGGAGAGCGA | Start: 56 |
| Tm | 60.514 | 59.091 | End: 175 |
| Size | 18 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | GTCAACCTGACCGCTCCG | GGCAATAAGGGAGAGCGATCA | Start: 43 |
| Tm | 60.433 | 59.929 | End: 173 |
| Size | 18 | 21 | 131 |
| Primer 4 details |
|---|
| Sequence | GTCAACCTGACCGCTCCG | TGGCAATAAGGGAGAGCGATC | Start: 43 |
| Tm | 60.433 | 59.929 | End: 174 |
| Size | 18 | 21 | 132 |
| Primer 5 details |
|---|
| Sequence | GTCAACCTGACCGCTCCG | TGGCAATAAGGGAGAGCGAT | Start: 43 |
| Tm | 60.433 | 58.868 | End: 174 |
| Size | 18 | 20 | 132 |
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