ssr_primer
Primer Details for TRINITY_DN75973_c4_g8_i1 |
|---|
| (T)14(G)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGCTGTTTGGCTCCTGTT | TTGGCTGCAGCATCACAAAC | Start: 713 |
| Tm | 60.107 | 59.968 | End: 902 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | TCCCTTGGCCGCAAATGTAT | AACAGGAGCCAAACAGCTGT | Start: 471 |
| Tm | 60.034 | 60.107 | End: 732 |
| Size | 20 | 20 | 262 |
| Primer 3 details |
|---|
| Sequence | AGCAGCATTTTCTCGCCTCT | AACAGGAGCCAAACAGCTGT | Start: 515 |
| Tm | 60.036 | 60.107 | End: 732 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | CCCTTGGCCGCAAATGTATG | AACAGGAGCCAAACAGCTGT | Start: 472 |
| Tm | 59.897 | 60.107 | End: 732 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TTCCTCACAGCTGTTTGGCT | TTGGCTGCAGCATCACAAAC | Start: 707 |
| Tm | 59.817 | 59.968 | End: 902 |
| Size | 20 | 20 | 196 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGCTGTTTGGCTCCTGTT | TTGGCTGCAGCATCACAAAC | Start: 713 |
| Tm | 60.107 | 59.968 | End: 902 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | TCCCTTGGCCGCAAATGTAT | AACAGGAGCCAAACAGCTGT | Start: 471 |
| Tm | 60.034 | 60.107 | End: 732 |
| Size | 20 | 20 | 262 |
| Primer 3 details |
|---|
| Sequence | AGCAGCATTTTCTCGCCTCT | AACAGGAGCCAAACAGCTGT | Start: 515 |
| Tm | 60.036 | 60.107 | End: 732 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | CCCTTGGCCGCAAATGTATG | AACAGGAGCCAAACAGCTGT | Start: 472 |
| Tm | 59.897 | 60.107 | End: 732 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TTCCTCACAGCTGTTTGGCT | TTGGCTGCAGCATCACAAAC | Start: 707 |
| Tm | 59.817 | 59.968 | End: 902 |
| Size | 20 | 20 | 196 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGCTGTTTGGCTCCTGTT | TTGGCTGCAGCATCACAAAC | Start: 713 |
| Tm | 60.107 | 59.968 | End: 902 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | TCCCTTGGCCGCAAATGTAT | AACAGGAGCCAAACAGCTGT | Start: 471 |
| Tm | 60.034 | 60.107 | End: 732 |
| Size | 20 | 20 | 262 |
| Primer 3 details |
|---|
| Sequence | AGCAGCATTTTCTCGCCTCT | AACAGGAGCCAAACAGCTGT | Start: 515 |
| Tm | 60.036 | 60.107 | End: 732 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | CCCTTGGCCGCAAATGTATG | AACAGGAGCCAAACAGCTGT | Start: 472 |
| Tm | 59.897 | 60.107 | End: 732 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TTCCTCACAGCTGTTTGGCT | TTGGCTGCAGCATCACAAAC | Start: 707 |
| Tm | 59.817 | 59.968 | End: 902 |
| Size | 20 | 20 | 196 |
(C)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAGCTGTTTGGCTCCTGTT | TTGGCTGCAGCATCACAAAC | Start: 713 |
| Tm | 60.107 | 59.968 | End: 902 |
| Size | 20 | 20 | 190 |
| Primer 2 details |
|---|
| Sequence | TCCCTTGGCCGCAAATGTAT | AACAGGAGCCAAACAGCTGT | Start: 471 |
| Tm | 60.034 | 60.107 | End: 732 |
| Size | 20 | 20 | 262 |
| Primer 3 details |
|---|
| Sequence | AGCAGCATTTTCTCGCCTCT | AACAGGAGCCAAACAGCTGT | Start: 515 |
| Tm | 60.036 | 60.107 | End: 732 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | CCCTTGGCCGCAAATGTATG | AACAGGAGCCAAACAGCTGT | Start: 472 |
| Tm | 59.897 | 60.107 | End: 732 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TTCCTCACAGCTGTTTGGCT | TTGGCTGCAGCATCACAAAC | Start: 707 |
| Tm | 59.817 | 59.968 | End: 902 |
| Size | 20 | 20 | 196 |
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