ssr_primer
Primer Details for TRINITY_DN75965_c6_g1_i7 |
|---|
| (G)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTGGAGACACAGCCATGG | TTCAGCGGCTTGAAAACGTG | Start: 322 |
| Tm | 59.963 | 59.971 | End: 429 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | ACTTGGAGACACAGCCATGG | AGCGGCTTGAAAACGTGTTC | Start: 322 |
| Tm | 59.963 | 59.971 | End: 426 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | ACTTGGAGACACAGCCATGG | TGCTTCAGCGGCTTGAAAAC | Start: 322 |
| Tm | 59.963 | 59.969 | End: 432 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | CGCAACGCCAGAAGTTACAG | ATCGGTCAGTTCGGGGACTA | Start: 482 |
| Tm | 59.836 | 60.034 | End: 713 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CGCAACGCCAGAAGTTACAG | AGAGTGGCCAAGAACATCGG | Start: 482 |
| Tm | 59.836 | 60.036 | End: 728 |
| Size | 20 | 20 | 247 |
(T)26: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTGGAGACACAGCCATGG | TTCAGCGGCTTGAAAACGTG | Start: 322 |
| Tm | 59.963 | 59.971 | End: 429 |
| Size | 20 | 20 | 108 |
| Primer 2 details |
|---|
| Sequence | ACTTGGAGACACAGCCATGG | AGCGGCTTGAAAACGTGTTC | Start: 322 |
| Tm | 59.963 | 59.971 | End: 426 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | ACTTGGAGACACAGCCATGG | TGCTTCAGCGGCTTGAAAAC | Start: 322 |
| Tm | 59.963 | 59.969 | End: 432 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | CGCAACGCCAGAAGTTACAG | ATCGGTCAGTTCGGGGACTA | Start: 482 |
| Tm | 59.836 | 60.034 | End: 713 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CGCAACGCCAGAAGTTACAG | AGAGTGGCCAAGAACATCGG | Start: 482 |
| Tm | 59.836 | 60.036 | End: 728 |
| Size | 20 | 20 | 247 |
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