ssr_primer
Primer Details for TRINITY_DN75955_c1_g2_i4 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAAACACCCTCAAGCCCA | AACTCACGCTCTTCTGGTGG | Start: 985 |
| Tm | 60.033 | 59.966 | End: 1251 |
| Size | 20 | 20 | 267 |
| Primer 2 details |
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| Sequence | CCACCAGAAGAGCGTGAGTT | GGTGGCGGTCTCTTCTTTGA | Start: 1232 |
| Tm | 59.966 | 59.965 | End: 1405 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
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| Sequence | CCACCAGAAGAGCGTGAGTT | AGGTGGCGGTCTCTTCTTTG | Start: 1232 |
| Tm | 59.966 | 59.965 | End: 1406 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
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| Sequence | CCCGTAGACACCTAATCCGC | CAGTGCTGGGACAATTTGGC | Start: 426 |
| Tm | 59.968 | 60.038 | End: 625 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
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| Sequence | CCCGTAGACACCTAATCCGC | GCAGTGCTGGGACAATTTGG | Start: 426 |
| Tm | 59.968 | 60.038 | End: 626 |
| Size | 20 | 20 | 201 |
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