ssr_primer
Primer Details for TRINITY_DN75948_c0_g2_i5 |
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| (A)26gaaaaaaatcccctcttctttcccctaaacaatt(A)34: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCTCCGGTGCAGTGAATC | CTCCGACCACCTCTCCTACA | Start: 2229 |
| Tm | 60.036 | 60.034 | End: 2346 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
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| Sequence | GCTTGCGGACGTGGATTTTT | AACTTGCCTGAGATGTCGCA | Start: 422 |
| Tm | 60.04 | 59.965 | End: 537 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
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| Sequence | CACTCAGACGTGCTGGCTAA | ATCAGTGGAACGTGTGCAGT | Start: 905 |
| Tm | 60.038 | 59.893 | End: 1151 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
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| Sequence | CACTCAGACGTGCTGGCTAA | TGGAACGTGTGCAGTGTCTT | Start: 905 |
| Tm | 60.038 | 60.108 | End: 1146 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
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| Sequence | GCTTGCGGACGTGGATTTTT | GCTGCAAGATGTGAGCAACC | Start: 422 |
| Tm | 60.04 | 60.109 | End: 584 |
| Size | 20 | 20 | 163 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCTCCGGTGCAGTGAATC | CTCCGACCACCTCTCCTACA | Start: 2229 |
| Tm | 60.036 | 60.034 | End: 2346 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | GCTTGCGGACGTGGATTTTT | AACTTGCCTGAGATGTCGCA | Start: 422 |
| Tm | 60.04 | 59.965 | End: 537 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | CACTCAGACGTGCTGGCTAA | ATCAGTGGAACGTGTGCAGT | Start: 905 |
| Tm | 60.038 | 59.893 | End: 1151 |
| Size | 20 | 20 | 247 |
| Primer 4 details |
|---|
| Sequence | CACTCAGACGTGCTGGCTAA | TGGAACGTGTGCAGTGTCTT | Start: 905 |
| Tm | 60.038 | 60.108 | End: 1146 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | GCTTGCGGACGTGGATTTTT | GCTGCAAGATGTGAGCAACC | Start: 422 |
| Tm | 60.04 | 60.109 | End: 584 |
| Size | 20 | 20 | 163 |
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