ssr_primer
Primer Details for TRINITY_DN75916_c0_g2_i3 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAATTGCCGGCACTCATCT | GTCGAAGTGGAAGCAGGACA | Start: 306 |
| Tm | 60.035 | 59.966 | End: 465 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | ACCGTTTGGCGTCTACCATT | GTCGAAGTGGAAGCAGGACA | Start: 327 |
| Tm | 59.964 | 59.966 | End: 465 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
|---|
| Sequence | TTGTCCGGGTGATCTCAACG | GTCGAAGTGGAAGCAGGACA | Start: 190 |
| Tm | 60.038 | 59.966 | End: 465 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TTGTCCGGGTGATCTCAACG | AGATGAGTGCCGGCAATTGA | Start: 190 |
| Tm | 60.038 | 60.035 | End: 325 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | TCAATTGCCGGCACTCATCT | GTCACCAGGAGTCGAAGTGG | Start: 306 |
| Tm | 60.035 | 60.038 | End: 475 |
| Size | 20 | 20 | 170 |
(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAATTGCCGGCACTCATCT | GTCGAAGTGGAAGCAGGACA | Start: 306 |
| Tm | 60.035 | 59.966 | End: 465 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | ACCGTTTGGCGTCTACCATT | GTCGAAGTGGAAGCAGGACA | Start: 327 |
| Tm | 59.964 | 59.966 | End: 465 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
|---|
| Sequence | TTGTCCGGGTGATCTCAACG | GTCGAAGTGGAAGCAGGACA | Start: 190 |
| Tm | 60.038 | 59.966 | End: 465 |
| Size | 20 | 20 | 276 |
| Primer 4 details |
|---|
| Sequence | TTGTCCGGGTGATCTCAACG | AGATGAGTGCCGGCAATTGA | Start: 190 |
| Tm | 60.038 | 60.035 | End: 325 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | TCAATTGCCGGCACTCATCT | GTCACCAGGAGTCGAAGTGG | Start: 306 |
| Tm | 60.035 | 60.038 | End: 475 |
| Size | 20 | 20 | 170 |
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