ssr_primer
Primer Details for TRINITY_DN75862_c0_g1_i1 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGATCGGGAGCAAGATGA | TGGTATAGGCGGCCTTTTCC | Start: 246 |
| Tm | 59.894 | 59.818 | End: 366 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
|---|
| Sequence | GGGTCCCAAGCCTCCAATTT | TCATCTTGCTCCCGATCTGC | Start: 113 |
| Tm | 60.252 | 59.894 | End: 265 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
|---|
| Sequence | CACCACCAGGAGTTGTCCAA | TGTGGCTTGACGACTCAACA | Start: 1390 |
| Tm | 59.817 | 59.823 | End: 1575 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | CACCACCAGGAGTTGTCCAA | TGAACAGTGTGGCTTGACGA | Start: 1390 |
| Tm | 59.817 | 59.823 | End: 1582 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
|---|
| Sequence | CACCACCAGGAGTTGTCCAA | AACAGTGTGGCTTGACGACT | Start: 1390 |
| Tm | 59.817 | 59.822 | End: 1580 |
| Size | 20 | 20 | 191 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGATCGGGAGCAAGATGA | TGGTATAGGCGGCCTTTTCC | Start: 246 |
| Tm | 59.894 | 59.818 | End: 366 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
|---|
| Sequence | GGGTCCCAAGCCTCCAATTT | TCATCTTGCTCCCGATCTGC | Start: 113 |
| Tm | 60.252 | 59.894 | End: 265 |
| Size | 20 | 20 | 153 |
| Primer 3 details |
|---|
| Sequence | CACCACCAGGAGTTGTCCAA | TGTGGCTTGACGACTCAACA | Start: 1390 |
| Tm | 59.817 | 59.823 | End: 1575 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | CACCACCAGGAGTTGTCCAA | TGAACAGTGTGGCTTGACGA | Start: 1390 |
| Tm | 59.817 | 59.823 | End: 1582 |
| Size | 20 | 20 | 193 |
| Primer 5 details |
|---|
| Sequence | CACCACCAGGAGTTGTCCAA | AACAGTGTGGCTTGACGACT | Start: 1390 |
| Tm | 59.817 | 59.822 | End: 1580 |
| Size | 20 | 20 | 191 |
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