ssr_primer
Primer Details for TRINITY_DN75855_c0_g1_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGCAGCTCTACGCAAATTC | GATTGCACAGGGTCTGCTCT | Start: 1313 |
| Tm | 59.973 | 60.036 | End: 1474 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
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| Sequence | GATGCACCATGGCGATTTCC | ACGATAAACTGGCTCGGCAA | Start: 337 |
| Tm | 59.969 | 60.037 | End: 467 |
| Size | 20 | 20 | 131 |
| Primer 3 details |
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| Sequence | GATGCACCATGGCGATTTCC | CGGGCATGGTTTTCTTGTGG | Start: 337 |
| Tm | 59.969 | 60.038 | End: 560 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
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| Sequence | TTGCCGAGCCAGTTTATCGT | CACCTTGAGAGTCTTGGCGT | Start: 448 |
| Tm | 60.037 | 59.966 | End: 703 |
| Size | 20 | 20 | 256 |
| Primer 5 details |
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| Sequence | CCACAAGAAAACCATGCCCG | CACCTTGAGAGTCTTGGCGT | Start: 541 |
| Tm | 60.038 | 59.966 | End: 703 |
| Size | 20 | 20 | 163 |
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