ssr_primer
Primer Details for TRINITY_DN75734_c2_g1_i5 |
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| (A)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGCCGAGAACTCAATGAA | CGGAGCAGACTTGGTTTTGC | Start: 838 |
| Tm | 60.035 | 60.04 | End: 1057 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
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| Sequence | GTGACAAATACTGGGGCCGA | CGGAGCAGACTTGGTTTTGC | Start: 826 |
| Tm | 60.036 | 60.04 | End: 1057 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
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| Sequence | GGCTTCAAGGCTGCTCTGTA | CGGAGCAGACTTGGTTTTGC | Start: 861 |
| Tm | 60.036 | 60.04 | End: 1057 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
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| Sequence | AGGACAGTGAAATGCTGCCA | AAGGTGGTGTGCGCAAAATC | Start: 1369 |
| Tm | 59.889 | 59.968 | End: 1531 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
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| Sequence | GGGGCCGAGAACTCAATGAA | CAGGGGAGCTGTCATCATCC | Start: 838 |
| Tm | 60.035 | 59.892 | End: 1116 |
| Size | 20 | 20 | 279 |
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