ssr_primer
Primer Details for TRINITY_DN75688_c19_g1_i1 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCACGGTTTTAAAGGCGG | TGAAGGATACAGCGAGCAGA | Start: 69 |
| Tm | 59.969 | 58.526 | End: 195 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
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| Sequence | TTGCACGGTTTTAAAGGCGG | AGGATACAGCGAGCAGAAACA | Start: 69 |
| Tm | 59.969 | 59.449 | End: 192 |
| Size | 20 | 21 | 124 |
| Primer 3 details |
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| Sequence | AAGGCGGGGTCTAAAAACGT | TGAAGGATACAGCGAGCAGA | Start: 82 |
| Tm | 59.891 | 58.526 | End: 195 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | AAGGCGGGGTCTAAAAACGT | AGGATACAGCGAGCAGAAACA | Start: 82 |
| Tm | 59.891 | 59.449 | End: 192 |
| Size | 20 | 21 | 111 |
| Primer 5 details |
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| Sequence | TTGCACGGTTTTAAAGGCGG | TGAAGGATACAGCGAGCAGAA | Start: 69 |
| Tm | 59.969 | 59.175 | End: 195 |
| Size | 20 | 21 | 127 |
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