ssr_primer
Primer Details for TRINITY_DN75684_c0_g5_i2 |
|---|
| (TGT)5tgctgctgc(TGT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGTTCTGAGACGTTTGGG | AAACGCCACCAGAGGTAGTG | Start: 214 |
| Tm | 59.967 | 59.965 | End: 320 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
|---|
| Sequence | CGGAACTACAGGGTGCACTT | TTTCTGTAGCAGCGCAGACA | Start: 1522 |
| Tm | 59.965 | 59.967 | End: 1630 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | TCCGTTCTGAGACGTTTGGG | TGCAAGCCTACATCTGTGCA | Start: 214 |
| Tm | 59.967 | 59.963 | End: 435 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | CACTACCTCTGGTGGCGTTT | TGCAAGCCTACATCTGTGCA | Start: 301 |
| Tm | 59.965 | 59.963 | End: 435 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | TGGGTTCATGCCTAGCTGTG | TGCAAGCCTACATCTGTGCA | Start: 230 |
| Tm | 60.035 | 59.963 | End: 435 |
| Size | 20 | 20 | 206 |
(T)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGTTCTGAGACGTTTGGG | AAACGCCACCAGAGGTAGTG | Start: 214 |
| Tm | 59.967 | 59.965 | End: 320 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
|---|
| Sequence | CGGAACTACAGGGTGCACTT | TTTCTGTAGCAGCGCAGACA | Start: 1522 |
| Tm | 59.965 | 59.967 | End: 1630 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | TCCGTTCTGAGACGTTTGGG | TGCAAGCCTACATCTGTGCA | Start: 214 |
| Tm | 59.967 | 59.963 | End: 435 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | CACTACCTCTGGTGGCGTTT | TGCAAGCCTACATCTGTGCA | Start: 301 |
| Tm | 59.965 | 59.963 | End: 435 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | TGGGTTCATGCCTAGCTGTG | TGCAAGCCTACATCTGTGCA | Start: 230 |
| Tm | 60.035 | 59.963 | End: 435 |
| Size | 20 | 20 | 206 |
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