ssr_primer
Primer Details for TRINITY_DN75681_c8_g2_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAGCATTTTAGGCCACGT | CTCGGCTCTTGTTCGATCGA | Start: 152 |
| Tm | 59.962 | 59.901 | End: 279 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | TGGAGCATTTTAGGCCACGT | ACCTCGGCTCTTGTTCGATC | Start: 152 |
| Tm | 59.962 | 59.826 | End: 281 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
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| Sequence | TGGAGCATTTTAGGCCACGT | ATAATTTTTGAGCGCGGCCG | Start: 152 |
| Tm | 59.962 | 60.248 | End: 391 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
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| Sequence | TCGATCGAACAAGAGCCGAG | ATAATTTTTGAGCGCGGCCG | Start: 260 |
| Tm | 59.901 | 60.248 | End: 391 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
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| Sequence | CGTGGAAAAATAGCGGCTCC | ACGTGGCCTAAAATGCTCCA | Start: 33 |
| Tm | 59.623 | 59.962 | End: 171 |
| Size | 20 | 20 | 139 |
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