ssr_primer
Primer Details for TRINITY_DN75665_c5_g2_i8 |
|---|
| (C)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTCTCTTTGCAGCAAGGT | CCACGGTTGGTCGAGAGAAT | Start: 1143 |
| Tm | 59.89 | 59.753 | End: 1399 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | ATCTTGGAGCGAGGACACAC | ACCTTGCTGCAAAGAGAGCT | Start: 901 |
| Tm | 59.752 | 59.89 | End: 1162 |
| Size | 20 | 20 | 262 |
| Primer 3 details |
|---|
| Sequence | GCTGATGATGGGTAGGGCTG | ACCTTGCTGCAAAGAGAGCT | Start: 962 |
| Tm | 60.251 | 59.89 | End: 1162 |
| Size | 20 | 20 | 201 |
| Primer 4 details |
|---|
| Sequence | CGTTCCTCCTCAGCACGTAG | TCGAGGCTGTTTATTGGGGG | Start: 150 |
| Tm | 60.179 | 59.745 | End: 293 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | ATCTTGGAGCGAGGACACAC | GGCTCCGTGACAGACAACTT | Start: 901 |
| Tm | 59.752 | 60.25 | End: 1118 |
| Size | 20 | 20 | 218 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTCTCTTTGCAGCAAGGT | CCACGGTTGGTCGAGAGAAT | Start: 1143 |
| Tm | 59.89 | 59.753 | End: 1399 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
|---|
| Sequence | ATCTTGGAGCGAGGACACAC | ACCTTGCTGCAAAGAGAGCT | Start: 901 |
| Tm | 59.752 | 59.89 | End: 1162 |
| Size | 20 | 20 | 262 |
| Primer 3 details |
|---|
| Sequence | GCTGATGATGGGTAGGGCTG | ACCTTGCTGCAAAGAGAGCT | Start: 962 |
| Tm | 60.251 | 59.89 | End: 1162 |
| Size | 20 | 20 | 201 |
| Primer 4 details |
|---|
| Sequence | CGTTCCTCCTCAGCACGTAG | TCGAGGCTGTTTATTGGGGG | Start: 150 |
| Tm | 60.179 | 59.745 | End: 293 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | ATCTTGGAGCGAGGACACAC | GGCTCCGTGACAGACAACTT | Start: 901 |
| Tm | 59.752 | 60.25 | End: 1118 |
| Size | 20 | 20 | 218 |
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