ssr_primer
Primer Details for TRINITY_DN75649_c1_g3_i2 |
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| (C)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTTCCTGTGACCTGTGGG | TGACTCGAAACGGGCAATCA | Start: 27 |
| Tm | 59.817 | 59.966 | End: 219 |
| Size | 20 | 20 | 193 |
| Primer 2 details |
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| Sequence | GGGAGTCAGGAGGTACCAGT | TGCCTGCATTTTGGTGGGTA | Start: 1538 |
| Tm | 59.959 | 60.179 | End: 1772 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
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| Sequence | TGATTGCCCGTTTCGAGTCA | CCCCAGGAAATCAAGGCACA | Start: 200 |
| Tm | 59.966 | 60.252 | End: 397 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
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| Sequence | GGGAGTCAGGAGGTACCAGT | GCCTGCATTTTGGTGGGTAC | Start: 1538 |
| Tm | 59.959 | 59.754 | End: 1771 |
| Size | 20 | 20 | 234 |
| Primer 5 details |
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| Sequence | TTGTTCCTGTGACCTGTGGG | GTGAGGCCTTTGGTGAGGAA | Start: 27 |
| Tm | 59.817 | 59.89 | End: 286 |
| Size | 20 | 20 | 260 |
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