ssr_primer
Primer Details for TRINITY_DN75649_c1_g2_i2 |
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| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGGTTGCGCTGTCACAAA | GATTGAAGCGCTGGATGCAG | Start: 541 |
| Tm | 59.9 | 59.971 | End: 772 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
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| Sequence | CTTGGTTGCGCTGTCACAAA | GAAGCGCTGGATGCAGAAAG | Start: 541 |
| Tm | 59.9 | 59.902 | End: 768 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
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| Sequence | TGAAGCGTTGGGTGCAGTAA | GATTGAAGCGCTGGATGCAG | Start: 512 |
| Tm | 60.179 | 59.971 | End: 772 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
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| Sequence | TTGAAGCGTTGGGTGCAGTA | GATTGAAGCGCTGGATGCAG | Start: 511 |
| Tm | 60.179 | 59.971 | End: 772 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
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| Sequence | CTTGGTTGCGCTGTCACAAA | CCCCAGAAGCTCCACTAACG | Start: 541 |
| Tm | 59.9 | 60.108 | End: 728 |
| Size | 20 | 20 | 188 |
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