ssr_primer
Primer Details for TRINITY_DN75627_c4_g1_i4 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATGTTGACCGAGACCCACA | TGCTGAATTTGGCTCGGACT | Start: 1431 |
| Tm | 59.965 | 59.963 | End: 1578 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | CGGTGGACATGGATGTTGGA | AGGCTCCACTCGACACAATG | Start: 2572 |
| Tm | 60.036 | 60.037 | End: 2823 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | GGTCTCGGATAGCGATGTGG | TCCAACATCCATGTCCACCG | Start: 2360 |
| Tm | 60.039 | 60.036 | End: 2591 |
| Size | 20 | 20 | 232 |
| Primer 4 details |
|---|
| Sequence | GGTCTCGGATAGCGATGTGG | GAGGGTTCCTTCACGACAGG | Start: 2360 |
| Tm | 60.039 | 60.037 | End: 2551 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
|---|
| Sequence | CAACGGCAGGAAGTTTTGCA | TCCAACATCCATGTCCACCG | Start: 2316 |
| Tm | 59.898 | 60.036 | End: 2591 |
| Size | 20 | 20 | 276 |
(A)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATGTTGACCGAGACCCACA | TGCTGAATTTGGCTCGGACT | Start: 1431 |
| Tm | 59.965 | 59.963 | End: 1578 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | CGGTGGACATGGATGTTGGA | AGGCTCCACTCGACACAATG | Start: 2572 |
| Tm | 60.036 | 60.037 | End: 2823 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
|---|
| Sequence | GGTCTCGGATAGCGATGTGG | TCCAACATCCATGTCCACCG | Start: 2360 |
| Tm | 60.039 | 60.036 | End: 2591 |
| Size | 20 | 20 | 232 |
| Primer 4 details |
|---|
| Sequence | GGTCTCGGATAGCGATGTGG | GAGGGTTCCTTCACGACAGG | Start: 2360 |
| Tm | 60.039 | 60.037 | End: 2551 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
|---|
| Sequence | CAACGGCAGGAAGTTTTGCA | TCCAACATCCATGTCCACCG | Start: 2316 |
| Tm | 59.898 | 60.036 | End: 2591 |
| Size | 20 | 20 | 276 |
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