ssr_primer
Primer Details for TRINITY_DN75621_c3_g1_i1 |
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| (T)24(G)16ctgggttgggtgtg(T)29: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGTGACGGTCGTGAGAGA | CTCGTTTTTCCCTGTTGGCG | Start: 306 |
| Tm | 59.965 | 60.04 | End: 476 |
| Size | 20 | 20 | 171 |
| Primer 2 details |
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| Sequence | ATGAACGAAGACGGCATGGT | CTCGTTTTTCCCTGTTGGCG | Start: 240 |
| Tm | 60.036 | 60.04 | End: 476 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
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| Sequence | TTCTTTTTGTGGCTGCCTGC | TCTCTCACGACCGTCACTCT | Start: 100 |
| Tm | 59.895 | 59.965 | End: 325 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
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| Sequence | TTCTTTTTGTGGCTGCCTGC | ACCATGCCGTCTTCGTTCAT | Start: 100 |
| Tm | 59.895 | 60.036 | End: 259 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
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| Sequence | ATGAACGAAGACGGCATGGT | GAGAGGAGGCAGCGTTGAAT | Start: 240 |
| Tm | 60.036 | 60.108 | End: 353 |
| Size | 20 | 20 | 114 |
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