ssr_primer
Primer Details for TRINITY_DN75591_c3_g2_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTGCCTATGCGGTGGAAT | CGATACCTTATCTGGGGCCG | Start: 65 |
| Tm | 60.035 | 59.753 | End: 334 |
| Size | 20 | 20 | 270 |
| Primer 2 details |
|---|
| Sequence | CGGCCCCAGATAAGGTATCG | GGGCAGAATCCAGTATGCGA | Start: 315 |
| Tm | 59.753 | 59.893 | End: 430 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | CGGCCCCAGATAAGGTATCG | AGGGCAGAATCCAGTATGCG | Start: 315 |
| Tm | 59.753 | 59.893 | End: 431 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | GTTTGTTGCCTATGCGGTGG | CGATACCTTATCTGGGGCCG | Start: 62 |
| Tm | 60.109 | 59.753 | End: 334 |
| Size | 20 | 20 | 273 |
| Primer 5 details |
|---|
| Sequence | GCGCCTGATTTAGCCACTTC | GGGCAGAATCCAGTATGCGA | Start: 283 |
| Tm | 59.621 | 59.893 | End: 430 |
| Size | 20 | 20 | 148 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTGCCTATGCGGTGGAAT | CGATACCTTATCTGGGGCCG | Start: 65 |
| Tm | 60.035 | 59.753 | End: 334 |
| Size | 20 | 20 | 270 |
| Primer 2 details |
|---|
| Sequence | CGGCCCCAGATAAGGTATCG | GGGCAGAATCCAGTATGCGA | Start: 315 |
| Tm | 59.753 | 59.893 | End: 430 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | CGGCCCCAGATAAGGTATCG | AGGGCAGAATCCAGTATGCG | Start: 315 |
| Tm | 59.753 | 59.893 | End: 431 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | GTTTGTTGCCTATGCGGTGG | CGATACCTTATCTGGGGCCG | Start: 62 |
| Tm | 60.109 | 59.753 | End: 334 |
| Size | 20 | 20 | 273 |
| Primer 5 details |
|---|
| Sequence | GCGCCTGATTTAGCCACTTC | GGGCAGAATCCAGTATGCGA | Start: 283 |
| Tm | 59.621 | 59.893 | End: 430 |
| Size | 20 | 20 | 148 |
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