Primer Details for TRINITY_DN75588_c4_g1_i1 |
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| (AAG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAAAACACTCAAGAGCGCGC | CCGTCATCATCATCATCTTTACTGT | Start: 61 |
| Tm | 60.317 | 59.012 | End: 161 |
| Size | 20 | 25 | 101 |
| Primer 2 details |
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| Sequence | AAAAACACTCAAGAGCGCGC | TCCGTCATCATCATCATCTTTACTGT | Start: 61 |
| Tm | 60.317 | 60.183 | End: 162 |
| Size | 20 | 26 | 102 |
| Primer 3 details |
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| Sequence | AAAAACACTCAAGAGCGCGC | TCCGTCATCATCATCATCTTTACTG | Start: 61 |
| Tm | 60.317 | 58.781 | End: 162 |
| Size | 20 | 25 | 102 |
| Primer 4 details |
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| Sequence | AAAAACACTCAAGAGCGCGC | TCCGTCATCATCATCATCTTTACT | Start: 61 |
| Tm | 60.317 | 57.589 | End: 162 |
| Size | 20 | 24 | 102 |
| Primer 5 details |
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| Sequence | AAAAACACTCAAGAGCGCGC | CCGTCATCATCATCATCTTTACTG | Start: 61 |
| Tm | 60.317 | 57.537 | End: 161 |
| Size | 20 | 24 | 101 |
