ssr_primer
Primer Details for TRINITY_DN75556_c5_g1_i1 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTCTGCTATTTGGCGAGA | AAAGGAGAGGAGAGGGGACG | Start: 24 |
| Tm | 59.534 | 60.325 | End: 243 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
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| Sequence | CCCTCTGCTATTTGGCGAGA | GAGGAGAGGGGACGCTTTTT | Start: 24 |
| Tm | 59.534 | 59.675 | End: 237 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
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| Sequence | CTGGTTGTTGTTTTCCGCCG | AAAGGAGAGGAGAGGGGACG | Start: 70 |
| Tm | 60.594 | 60.325 | End: 243 |
| Size | 20 | 20 | 174 |
| Primer 4 details |
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| Sequence | CTGGTTGTTGTTTTCCGCCG | GAGGAGAGGGGACGCTTTTT | Start: 70 |
| Tm | 60.594 | 59.675 | End: 237 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
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| Sequence | CCCTCTGCTATTTGGCGAGA | AGAGGAGAGGGGACGCTTTT | Start: 24 |
| Tm | 59.534 | 60.546 | End: 238 |
| Size | 20 | 20 | 215 |
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