ssr_primer
Primer Details for TRINITY_DN75531_c1_g1_i1 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGGGAGTGTGTCAGACCC | CCGTGACCTTTTGGCTTTGG | Start: 68 |
| Tm | 59.528 | 59.968 | End: 171 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
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| Sequence | TTTTGGGAGTGTGTCAGACCC | CCGTGACCTTTTGGCTTTGG | Start: 67 |
| Tm | 60.134 | 59.968 | End: 171 |
| Size | 21 | 20 | 105 |
| Primer 3 details |
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| Sequence | TTTGGGAGTGTGTCAGACCC | CGTGACCTTTTGGCTTTGGT | Start: 68 |
| Tm | 59.528 | 59.256 | End: 170 |
| Size | 20 | 20 | 103 |
| Primer 4 details |
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| Sequence | TTTGGGAGTGTGTCAGACCC | ACCGTGACCTTTTGGCTTTG | Start: 68 |
| Tm | 59.528 | 59.256 | End: 172 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
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| Sequence | GGAGTGTGTCAGACCCCAC | CCGTGACCTTTTGGCTTTGG | Start: 72 |
| Tm | 59.628 | 59.968 | End: 171 |
| Size | 19 | 20 | 100 |
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