ssr_primer
Primer Details for TRINITY_DN75477_c3_g1_i1 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCACGTCTGTTGTGCTCC | TCAACTACGTCGGCAGCAAT | Start: 26 |
| Tm | 59.968 | 60.038 | End: 150 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
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| Sequence | TCTCACGTCTGTTGTGCTCC | TGCAGGGGGTGAGTCTATGA | Start: 26 |
| Tm | 59.968 | 59.957 | End: 261 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
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| Sequence | ATTGCTGCCGACGTAGTTGA | TGCAGGGGGTGAGTCTATGA | Start: 131 |
| Tm | 60.038 | 59.957 | End: 261 |
| Size | 20 | 20 | 131 |
| Primer 4 details |
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| Sequence | TCACGTCTGTTGTGCTCCAA | TCAACTACGTCGGCAGCAAT | Start: 28 |
| Tm | 59.823 | 60.038 | End: 150 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
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| Sequence | TGCTCCAACAGTGTGCTCTT | TCAACTACGTCGGCAGCAAT | Start: 40 |
| Tm | 59.819 | 60.038 | End: 150 |
| Size | 20 | 20 | 111 |
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