ssr_primer
Primer Details for TRINITY_DN75475_c5_g2_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCCTCCAAGAGCAAACTC | TTTACCAACGAGTCCTGGCC | Start: 415 |
| Tm | 59.963 | 59.964 | End: 635 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
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| Sequence | CGTGGAGAAAAGTTGGCTGC | TTTACCAACGAGTCCTGGCC | Start: 381 |
| Tm | 60.04 | 59.964 | End: 635 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
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| Sequence | GTGATAGGCTCCCAACCTCG | TTTACCAACGAGTCCTGGCC | Start: 439 |
| Tm | 59.895 | 59.964 | End: 635 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
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| Sequence | CGTGATAGGCTCCCAACCTC | TTTACCAACGAGTCCTGGCC | Start: 438 |
| Tm | 59.895 | 59.964 | End: 635 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
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| Sequence | AGGCCTCCAAGAGCAAACTC | CAAAGCCCGGGAGCATTTTC | Start: 415 |
| Tm | 59.963 | 60.109 | End: 557 |
| Size | 20 | 20 | 143 |
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