ssr_primer
Primer Details for TRINITY_DN75458_c8_g1_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGAATCCGACTGCCGTATC | CCCTAACTTCTCCCTCCCCT | Start: 161 |
| Tm | 59.97 | 59.656 | End: 325 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
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| Sequence | ACGAATCCGACTGCCGTATC | GGAGGAGTCGAGTGAAGAGC | Start: 161 |
| Tm | 59.97 | 59.546 | End: 434 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
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| Sequence | AGTAACGAATCCGACTGCCG | AAACTCATGCACAACACGCG | Start: 157 |
| Tm | 60.179 | 60.317 | End: 257 |
| Size | 20 | 20 | 101 |
| Primer 4 details |
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| Sequence | AGTAACGAATCCGACTGCCG | CCCTAACTTCTCCCTCCCCT | Start: 157 |
| Tm | 60.179 | 59.656 | End: 325 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
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| Sequence | AGTAACGAATCCGACTGCCG | GGAGGAGTCGAGTGAAGAGC | Start: 157 |
| Tm | 60.179 | 59.546 | End: 434 |
| Size | 20 | 20 | 278 |
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