ssr_primer
Primer Details for TRINITY_DN75448_c1_g4_i3 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAGCGGGAGGAGACACATC | ATAATTGCAGGGTCGGGTCC | Start: 483 |
| Tm | 60.179 | 59.817 | End: 605 |
| Size | 20 | 20 | 123 |
| Primer 2 details |
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| Sequence | ATAGCGAACGTTGCATGCAG | CCGTGCCGTATTAGAGGTCC | Start: 305 |
| Tm | 59.625 | 59.968 | End: 473 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
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| Sequence | ATAGCGAACGTTGCATGCAG | AGGTGAGAAGGAGCTGTTGC | Start: 305 |
| Tm | 59.625 | 59.964 | End: 532 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
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| Sequence | TTCAAAACCACTTTCGGGCG | AGGTGAGAAGGAGCTGTTGC | Start: 410 |
| Tm | 59.617 | 59.964 | End: 532 |
| Size | 20 | 20 | 123 |
| Primer 5 details |
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| Sequence | ATTCGTTTCCATCACCGCGA | CCGTGCCGTATTAGAGGTCC | Start: 355 |
| Tm | 60.391 | 59.968 | End: 473 |
| Size | 20 | 20 | 119 |
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