ssr_primer
Primer Details for TRINITY_DN75435_c0_g1_i5 |
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| (A)29: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCGGTTGGTAAGTCAGCGA | TGAGATCAGGTGGCTGGAGA | Start: 1717 |
| Tm | 60.039 | 59.958 | End: 1947 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
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| Sequence | GTCGGTTGGTAAGTCAGCGA | GTGAGATCAGGTGGCTGGAG | Start: 1717 |
| Tm | 60.039 | 59.822 | End: 1948 |
| Size | 20 | 20 | 232 |
| Primer 3 details |
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| Sequence | TGTCGGTTGGTAAGTCAGCG | TGAGATCAGGTGGCTGGAGA | Start: 1716 |
| Tm | 60.319 | 59.958 | End: 1947 |
| Size | 20 | 20 | 232 |
| Primer 4 details |
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| Sequence | CTGGACCGACCTGAAGCAAT | CGGGCAGTGAACTGAGGTAC | Start: 3112 |
| Tm | 60.036 | 60.39 | End: 3347 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
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| Sequence | GGACCGACCTGAAGCAATCA | CGGGCAGTGAACTGAGGTAC | Start: 3114 |
| Tm | 60.036 | 60.39 | End: 3347 |
| Size | 20 | 20 | 234 |
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