ssr_primer
Primer Details for TRINITY_DN75363_c3_g1_i2 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCGAACTCTACGGCAGGA | GTTGGCGGGTAGAGTCTACG | Start: 663 |
| Tm | 60.036 | 59.9 | End: 856 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TCTCGAACTCTACGGCAGGA | CGAAGACAGCTGGACGATGT | Start: 663 |
| Tm | 60.036 | 60.109 | End: 906 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | CACACGTCCTTCCTCTTGCT | CGCTATGCCGCAATGAGATG | Start: 99 |
| Tm | 59.966 | 59.835 | End: 284 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | AATGCAAGTTTACGCCTCGC | AGCAAGAGGAAGGACGTGTG | Start: 3 |
| Tm | 59.832 | 59.966 | End: 118 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | GCCCACACGTTCGGATATCT | GTTGGCGGGTAGAGTCTACG | Start: 731 |
| Tm | 59.896 | 59.9 | End: 856 |
| Size | 20 | 20 | 126 |
(T)45: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCGAACTCTACGGCAGGA | GTTGGCGGGTAGAGTCTACG | Start: 663 |
| Tm | 60.036 | 59.9 | End: 856 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TCTCGAACTCTACGGCAGGA | CGAAGACAGCTGGACGATGT | Start: 663 |
| Tm | 60.036 | 60.109 | End: 906 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | CACACGTCCTTCCTCTTGCT | CGCTATGCCGCAATGAGATG | Start: 99 |
| Tm | 59.966 | 59.835 | End: 284 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | AATGCAAGTTTACGCCTCGC | AGCAAGAGGAAGGACGTGTG | Start: 3 |
| Tm | 59.832 | 59.966 | End: 118 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | GCCCACACGTTCGGATATCT | GTTGGCGGGTAGAGTCTACG | Start: 731 |
| Tm | 59.896 | 59.9 | End: 856 |
| Size | 20 | 20 | 126 |
(TAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCGAACTCTACGGCAGGA | GTTGGCGGGTAGAGTCTACG | Start: 663 |
| Tm | 60.036 | 59.9 | End: 856 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TCTCGAACTCTACGGCAGGA | CGAAGACAGCTGGACGATGT | Start: 663 |
| Tm | 60.036 | 60.109 | End: 906 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | CACACGTCCTTCCTCTTGCT | CGCTATGCCGCAATGAGATG | Start: 99 |
| Tm | 59.966 | 59.835 | End: 284 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | AATGCAAGTTTACGCCTCGC | AGCAAGAGGAAGGACGTGTG | Start: 3 |
| Tm | 59.832 | 59.966 | End: 118 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | GCCCACACGTTCGGATATCT | GTTGGCGGGTAGAGTCTACG | Start: 731 |
| Tm | 59.896 | 59.9 | End: 856 |
| Size | 20 | 20 | 126 |
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