ssr_primer
Primer Details for TRINITY_DN75344_c1_g1_i12 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGACGAAACACGCAAACA | CACACCTCATGATGCATGCG | Start: 996 |
| Tm | 59.975 | 59.971 | End: 1096 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ACGACGAAACACGCAAACAG | CACACCTCATGATGCATGCG | Start: 997 |
| Tm | 59.974 | 59.971 | End: 1096 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | CTGTGGAAAGCGTGTTTGGG | TCGAGACGATCTCTTTCGCG | Start: 1375 |
| Tm | 59.969 | 59.974 | End: 1572 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
|---|
| Sequence | TAGGACGACGAAACACGCAA | CACACCTCATGATGCATGCG | Start: 993 |
| Tm | 59.969 | 59.971 | End: 1096 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | ATGGTCGTAGAGTCCTGCCT | TCGAGACGATCTCTTTCGCG | Start: 1452 |
| Tm | 60.033 | 59.974 | End: 1572 |
| Size | 20 | 20 | 121 |
(T)30: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGACGAAACACGCAAACA | CACACCTCATGATGCATGCG | Start: 996 |
| Tm | 59.975 | 59.971 | End: 1096 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ACGACGAAACACGCAAACAG | CACACCTCATGATGCATGCG | Start: 997 |
| Tm | 59.974 | 59.971 | End: 1096 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | CTGTGGAAAGCGTGTTTGGG | TCGAGACGATCTCTTTCGCG | Start: 1375 |
| Tm | 59.969 | 59.974 | End: 1572 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
|---|
| Sequence | TAGGACGACGAAACACGCAA | CACACCTCATGATGCATGCG | Start: 993 |
| Tm | 59.969 | 59.971 | End: 1096 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | ATGGTCGTAGAGTCCTGCCT | TCGAGACGATCTCTTTCGCG | Start: 1452 |
| Tm | 60.033 | 59.974 | End: 1572 |
| Size | 20 | 20 | 121 |
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