ssr_primer
Primer Details for TRINITY_DN75343_c4_g4_i2 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGTGAGGATGAGTCGGGA | AGTGCTGTAGCCCTGGTCTA | Start: 1352 |
| Tm | 60.032 | 59.959 | End: 1500 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | CATGGTGAGGATGAGTCGGG | AGTGCTGTAGCCCTGGTCTA | Start: 1351 |
| Tm | 59.894 | 59.959 | End: 1500 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
|---|
| Sequence | ATGAGTTCTCAGAGGTGCGC | GGCAAATCATTCATGCGGCT | Start: 88 |
| Tm | 60.108 | 59.896 | End: 242 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | ATGAGTTCTCAGAGGTGCGC | AGGCAAATCATTCATGCGGC | Start: 88 |
| Tm | 60.108 | 59.896 | End: 243 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | CACTTTAAAGCGCGGCTCAG | ACCTTCGCAGTGCTAAACGA | Start: 542 |
| Tm | 60.179 | 59.967 | End: 700 |
| Size | 20 | 20 | 159 |
(A)11ctatcataaaaataaatggaggagcaacgaattaaaataaaccttattaatgggagtaac(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGTGAGGATGAGTCGGGA | AGTGCTGTAGCCCTGGTCTA | Start: 1352 |
| Tm | 60.032 | 59.959 | End: 1500 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | CATGGTGAGGATGAGTCGGG | AGTGCTGTAGCCCTGGTCTA | Start: 1351 |
| Tm | 59.894 | 59.959 | End: 1500 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
|---|
| Sequence | ATGAGTTCTCAGAGGTGCGC | GGCAAATCATTCATGCGGCT | Start: 88 |
| Tm | 60.108 | 59.896 | End: 242 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | ATGAGTTCTCAGAGGTGCGC | AGGCAAATCATTCATGCGGC | Start: 88 |
| Tm | 60.108 | 59.896 | End: 243 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | CACTTTAAAGCGCGGCTCAG | ACCTTCGCAGTGCTAAACGA | Start: 542 |
| Tm | 60.179 | 59.967 | End: 700 |
| Size | 20 | 20 | 159 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGTGAGGATGAGTCGGGA | AGTGCTGTAGCCCTGGTCTA | Start: 1352 |
| Tm | 60.032 | 59.959 | End: 1500 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | CATGGTGAGGATGAGTCGGG | AGTGCTGTAGCCCTGGTCTA | Start: 1351 |
| Tm | 59.894 | 59.959 | End: 1500 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
|---|
| Sequence | ATGAGTTCTCAGAGGTGCGC | GGCAAATCATTCATGCGGCT | Start: 88 |
| Tm | 60.108 | 59.896 | End: 242 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | ATGAGTTCTCAGAGGTGCGC | AGGCAAATCATTCATGCGGC | Start: 88 |
| Tm | 60.108 | 59.896 | End: 243 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | CACTTTAAAGCGCGGCTCAG | ACCTTCGCAGTGCTAAACGA | Start: 542 |
| Tm | 60.179 | 59.967 | End: 700 |
| Size | 20 | 20 | 159 |
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