ssr_primer
Primer Details for TRINITY_DN75316_c6_g1_i1 |
|---|
| (C)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGAAAGAACGGCGACTCA | CCTCTTTTCTTTCCGGCCCT | Start: 328 |
| Tm | 60.32 | 59.962 | End: 529 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | CTGGAAAGAACGGCGACTCA | ACGCAAAAGCATCGCACATT | Start: 328 |
| Tm | 60.32 | 60.039 | End: 462 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | CCGGCCTGCAAAATCGTTAG | TGAGTCGCCGTTCTTTCCAG | Start: 81 |
| Tm | 59.901 | 60.32 | End: 347 |
| Size | 20 | 20 | 267 |
| Primer 4 details |
|---|
| Sequence | CTGGAAAGAACGGCGACTCA | ACGAGCCTCTTCCCGTTTTT | Start: 328 |
| Tm | 60.32 | 59.892 | End: 495 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
|---|
| Sequence | CTGGAAAGAACGGCGACTCA | TGTAGCACGCAAAAGCATCG | Start: 328 |
| Tm | 60.32 | 59.834 | End: 468 |
| Size | 20 | 20 | 141 |
(G)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGAAAGAACGGCGACTCA | CCTCTTTTCTTTCCGGCCCT | Start: 328 |
| Tm | 60.32 | 59.962 | End: 529 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | CTGGAAAGAACGGCGACTCA | ACGCAAAAGCATCGCACATT | Start: 328 |
| Tm | 60.32 | 60.039 | End: 462 |
| Size | 20 | 20 | 135 |
| Primer 3 details |
|---|
| Sequence | CCGGCCTGCAAAATCGTTAG | TGAGTCGCCGTTCTTTCCAG | Start: 81 |
| Tm | 59.901 | 60.32 | End: 347 |
| Size | 20 | 20 | 267 |
| Primer 4 details |
|---|
| Sequence | CTGGAAAGAACGGCGACTCA | ACGAGCCTCTTCCCGTTTTT | Start: 328 |
| Tm | 60.32 | 59.892 | End: 495 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
|---|
| Sequence | CTGGAAAGAACGGCGACTCA | TGTAGCACGCAAAAGCATCG | Start: 328 |
| Tm | 60.32 | 59.834 | End: 468 |
| Size | 20 | 20 | 141 |
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