ssr_primer
Primer Details for TRINITY_DN75303_c6_g1_i1 |
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| (T)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATGAGCGCATTGGCAAAGT | GGGCTGGGTAGAACGACTTC | Start: 43 |
| Tm | 59.828 | 60.108 | End: 181 |
| Size | 20 | 20 | 139 |
| Primer 2 details |
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| Sequence | GCGTTTATAGAGCTTGCCGC | TATGTTTCGGCGGATTGGGG | Start: 101 |
| Tm | 60.042 | 60.465 | End: 240 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
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| Sequence | GATGAGCGCATTGGCAAAGT | TATGTTTCGGCGGATTGGGG | Start: 43 |
| Tm | 59.828 | 60.465 | End: 240 |
| Size | 20 | 20 | 198 |
| Primer 4 details |
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| Sequence | GCGTTTATAGAGCTTGCCGC | GGGGTCGGCCATCTTGAAAA | Start: 101 |
| Tm | 60.042 | 60.611 | End: 224 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
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| Sequence | GATGAGCGCATTGGCAAAGT | TATCCTAGTGCTCAGGGGCT | Start: 43 |
| Tm | 59.828 | 59.437 | End: 196 |
| Size | 20 | 20 | 154 |
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