ssr_primer
Primer Details for TRINITY_DN75303_c5_g3_i1 |
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| (G)15tggagggggcgatccgcggcgagcgc(G)16a(G)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCGAGACTCCAGATTCAA | TGAAGGCTCATACGGCGTTT | Start: 156 |
| Tm | 59.827 | 60.037 | End: 261 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
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| Sequence | CCGCGAGACTCCAGATTCAA | CGACGGAAATTTGGCAACGT | Start: 156 |
| Tm | 59.827 | 60.041 | End: 282 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
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| Sequence | GAGTTAACCCGCGAGACTCC | TGAAGGCTCATACGGCGTTT | Start: 148 |
| Tm | 60.179 | 60.037 | End: 261 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
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| Sequence | GGAGTTAACCCGCGAGACTC | TGAAGGCTCATACGGCGTTT | Start: 147 |
| Tm | 60.179 | 60.037 | End: 261 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
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| Sequence | GAGTTAACCCGCGAGACTCC | CGACGGAAATTTGGCAACGT | Start: 148 |
| Tm | 60.179 | 60.041 | End: 282 |
| Size | 20 | 20 | 135 |
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