ssr_primer
Primer Details for TRINITY_DN75303_c3_g1_i3 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCTAGTCAACTCGGGTTT | TCCAGTAAAACGCGACCACA | Start: 221 |
| Tm | 60.038 | 59.896 | End: 335 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | CCGCTAGTCAACTCGGGTTT | TGTCCAGTAAAACGCGACCA | Start: 221 |
| Tm | 60.038 | 59.896 | End: 337 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | TGGTCGCGTTTTACTGGACA | AAACCCGAGTTGACTAGCGG | Start: 126 |
| Tm | 59.896 | 60.038 | End: 240 |
| Size | 20 | 20 | 115 |
| Primer 4 details |
|---|
| Sequence | TGTGGTCGCGTTTTACTGGA | AAACCCGAGTTGACTAGCGG | Start: 124 |
| Tm | 59.896 | 60.038 | End: 240 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
|---|
| Sequence | CCGCTAGTCAACTCGGGTTT | TCCAGTAAAACGCGACCACA | Start: 29 |
| Tm | 60.038 | 59.896 | End: 143 |
| Size | 20 | 20 | 115 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCTAGTCAACTCGGGTTT | TCCAGTAAAACGCGACCACA | Start: 221 |
| Tm | 60.038 | 59.896 | End: 335 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | CCGCTAGTCAACTCGGGTTT | TGTCCAGTAAAACGCGACCA | Start: 221 |
| Tm | 60.038 | 59.896 | End: 337 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
|---|
| Sequence | TGGTCGCGTTTTACTGGACA | AAACCCGAGTTGACTAGCGG | Start: 126 |
| Tm | 59.896 | 60.038 | End: 240 |
| Size | 20 | 20 | 115 |
| Primer 4 details |
|---|
| Sequence | TGTGGTCGCGTTTTACTGGA | AAACCCGAGTTGACTAGCGG | Start: 124 |
| Tm | 59.896 | 60.038 | End: 240 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
|---|
| Sequence | CCGCTAGTCAACTCGGGTTT | TCCAGTAAAACGCGACCACA | Start: 29 |
| Tm | 60.038 | 59.896 | End: 143 |
| Size | 20 | 20 | 115 |
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