ssr_primer
Primer Details for TRINITY_DN75298_c1_g1_i3 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTGCTAAAACACGCCACAA | CGGAGAACTTTGACCACCGA | Start: 1527 |
| Tm | 59.97 | 59.967 | End: 1802 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
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| Sequence | AAGAGCGTGAACGGACACAT | TTGTGGCGTGTTTTAGCAGC | Start: 1423 |
| Tm | 59.966 | 59.97 | End: 1546 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
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| Sequence | CGAGCAAGAAATGAGCGACG | GTTGTTGACGGTGTGGATGC | Start: 89 |
| Tm | 59.976 | 60.04 | End: 358 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
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| Sequence | ATCCAGCACCACCAGCTAAC | TTGTGGCGTGTTTTAGCAGC | Start: 1288 |
| Tm | 60.035 | 59.97 | End: 1546 |
| Size | 20 | 20 | 259 |
| Primer 5 details |
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| Sequence | ATCCAGCACCACCAGCTAAC | ATGTGTCCGTTCACGCTCTT | Start: 1288 |
| Tm | 60.035 | 59.966 | End: 1442 |
| Size | 20 | 20 | 155 |
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