ssr_primer
Primer Details for TRINITY_DN75277_c3_g2_i4 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAGCCCAGCACATTCTTC | TTGGCTCGGATGGAATGCAT | Start: 1556 |
| Tm | 60.035 | 60.107 | End: 1757 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | AGCTGCCTGTGCTAAAGTGT | GAAGAATGTGCTGGGCTCCT | Start: 1462 |
| Tm | 59.891 | 60.035 | End: 1575 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | ATGCATTCCATCCGAGCCAA | ATGGTGGGCTTAGTCATGGC | Start: 1738 |
| Tm | 60.107 | 60.107 | End: 1908 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | ATGCATTCCATCCGAGCCAA | CTCGGGGCATCACATCTGTT | Start: 1738 |
| Tm | 60.107 | 60.108 | End: 1883 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | ATGCATTCCATCCGAGCCAA | GTCATGGCGATATCTCGGGG | Start: 1738 |
| Tm | 60.107 | 60.109 | End: 1896 |
| Size | 20 | 20 | 159 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGAGCCCAGCACATTCTTC | TTGGCTCGGATGGAATGCAT | Start: 1556 |
| Tm | 60.035 | 60.107 | End: 1757 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | AGCTGCCTGTGCTAAAGTGT | GAAGAATGTGCTGGGCTCCT | Start: 1462 |
| Tm | 59.891 | 60.035 | End: 1575 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | ATGCATTCCATCCGAGCCAA | ATGGTGGGCTTAGTCATGGC | Start: 1738 |
| Tm | 60.107 | 60.107 | End: 1908 |
| Size | 20 | 20 | 171 |
| Primer 4 details |
|---|
| Sequence | ATGCATTCCATCCGAGCCAA | CTCGGGGCATCACATCTGTT | Start: 1738 |
| Tm | 60.107 | 60.108 | End: 1883 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | ATGCATTCCATCCGAGCCAA | GTCATGGCGATATCTCGGGG | Start: 1738 |
| Tm | 60.107 | 60.109 | End: 1896 |
| Size | 20 | 20 | 159 |
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