ssr_primer
Primer Details for TRINITY_DN75221_c0_g1_i12 |
|---|
| (T)30: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCTAGGGCAGCATCAGT | GCCTAAGATCTCTGACGCCC | Start: 856 |
| Tm | 60.032 | 59.967 | End: 967 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
|---|
| Sequence | AGGATGCGGGAATTAGAGCG | ACTGATGCTGCCCTAGGAGA | Start: 736 |
| Tm | 59.966 | 60.032 | End: 875 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | TCGCGCCTTTTGTAAGAGGT | ACTGATGCTGCCCTAGGAGA | Start: 621 |
| Tm | 59.966 | 60.032 | End: 875 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | AGGATGCGGGAATTAGAGCG | GCCTAAGATCTCTGACGCCC | Start: 736 |
| Tm | 59.966 | 59.967 | End: 967 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AGGTGGTGGTCGGTGATTTC | ACTGATGCTGCCCTAGGAGA | Start: 637 |
| Tm | 59.964 | 60.032 | End: 875 |
| Size | 20 | 20 | 239 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCTAGGGCAGCATCAGT | GCCTAAGATCTCTGACGCCC | Start: 856 |
| Tm | 60.032 | 59.967 | End: 967 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
|---|
| Sequence | AGGATGCGGGAATTAGAGCG | ACTGATGCTGCCCTAGGAGA | Start: 736 |
| Tm | 59.966 | 60.032 | End: 875 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | TCGCGCCTTTTGTAAGAGGT | ACTGATGCTGCCCTAGGAGA | Start: 621 |
| Tm | 59.966 | 60.032 | End: 875 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | AGGATGCGGGAATTAGAGCG | GCCTAAGATCTCTGACGCCC | Start: 736 |
| Tm | 59.966 | 59.967 | End: 967 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AGGTGGTGGTCGGTGATTTC | ACTGATGCTGCCCTAGGAGA | Start: 637 |
| Tm | 59.964 | 60.032 | End: 875 |
| Size | 20 | 20 | 239 |
(T)11aatcgtcaggtttctggagaaaaacattcatagccatggagtaatatgttagcctctggtagaaatttcaaaattcaatcaatatccga(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCTAGGGCAGCATCAGT | GCCTAAGATCTCTGACGCCC | Start: 856 |
| Tm | 60.032 | 59.967 | End: 967 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
|---|
| Sequence | AGGATGCGGGAATTAGAGCG | ACTGATGCTGCCCTAGGAGA | Start: 736 |
| Tm | 59.966 | 60.032 | End: 875 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | TCGCGCCTTTTGTAAGAGGT | ACTGATGCTGCCCTAGGAGA | Start: 621 |
| Tm | 59.966 | 60.032 | End: 875 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | AGGATGCGGGAATTAGAGCG | GCCTAAGATCTCTGACGCCC | Start: 736 |
| Tm | 59.966 | 59.967 | End: 967 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AGGTGGTGGTCGGTGATTTC | ACTGATGCTGCCCTAGGAGA | Start: 637 |
| Tm | 59.964 | 60.032 | End: 875 |
| Size | 20 | 20 | 239 |
(T)12(G)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCCTAGGGCAGCATCAGT | GCCTAAGATCTCTGACGCCC | Start: 856 |
| Tm | 60.032 | 59.967 | End: 967 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
|---|
| Sequence | AGGATGCGGGAATTAGAGCG | ACTGATGCTGCCCTAGGAGA | Start: 736 |
| Tm | 59.966 | 60.032 | End: 875 |
| Size | 20 | 20 | 140 |
| Primer 3 details |
|---|
| Sequence | TCGCGCCTTTTGTAAGAGGT | ACTGATGCTGCCCTAGGAGA | Start: 621 |
| Tm | 59.966 | 60.032 | End: 875 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | AGGATGCGGGAATTAGAGCG | GCCTAAGATCTCTGACGCCC | Start: 736 |
| Tm | 59.966 | 59.967 | End: 967 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | AGGTGGTGGTCGGTGATTTC | ACTGATGCTGCCCTAGGAGA | Start: 637 |
| Tm | 59.964 | 60.032 | End: 875 |
| Size | 20 | 20 | 239 |
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