ssr_primer
Primer Details for TRINITY_DN75203_c0_g2_i5 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTGTCTGTGTGCGCATTG | CTCCGAGGAGAGGGAAGTGA | Start: 1406 |
| Tm | 59.971 | 60.034 | End: 1683 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | TCACTTCCCTCTCCTCGGAG | GAAGCCTGTCGAGAGTTGCT | Start: 1664 |
| Tm | 60.034 | 60.038 | End: 1834 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | CTGGGGACTTCAGTTCCACC | CTGGAATCCCACCACAGACC | Start: 337 |
| Tm | 59.963 | 60.035 | End: 477 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
|---|
| Sequence | CTGGGGACTTCAGTTCCACC | CCTGGAATCCCACCACAGAC | Start: 337 |
| Tm | 59.963 | 60.035 | End: 478 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | CCATGTTTTCTGGTGGTGCG | GTACCTCCTGCTGCATTGGT | Start: 557 |
| Tm | 60.039 | 60.035 | End: 824 |
| Size | 20 | 20 | 268 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTGTCTGTGTGCGCATTG | CTCCGAGGAGAGGGAAGTGA | Start: 1406 |
| Tm | 59.971 | 60.034 | End: 1683 |
| Size | 20 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | TCACTTCCCTCTCCTCGGAG | GAAGCCTGTCGAGAGTTGCT | Start: 1664 |
| Tm | 60.034 | 60.038 | End: 1834 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | CTGGGGACTTCAGTTCCACC | CTGGAATCCCACCACAGACC | Start: 337 |
| Tm | 59.963 | 60.035 | End: 477 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
|---|
| Sequence | CTGGGGACTTCAGTTCCACC | CCTGGAATCCCACCACAGAC | Start: 337 |
| Tm | 59.963 | 60.035 | End: 478 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
|---|
| Sequence | CCATGTTTTCTGGTGGTGCG | GTACCTCCTGCTGCATTGGT | Start: 557 |
| Tm | 60.039 | 60.035 | End: 824 |
| Size | 20 | 20 | 268 |
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