ssr_primer
Primer Details for TRINITY_DN75171_c2_g1_i1 |
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| (C)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACCTCGTGTTTGTGGCAAG | ATGACCTTCTTGCAGGAGCC | Start: 2970 |
| Tm | 59.971 | 60.035 | End: 3154 |
| Size | 20 | 20 | 185 |
| Primer 2 details |
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| Sequence | TTCAGCGCATGTTCCAGGAT | AGGAACGATTGCTGGAAGGG | Start: 1779 |
| Tm | 60.035 | 60.035 | End: 1926 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
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| Sequence | TGCGCGGATTGGTAAGAAGT | TTCTGACTTTTCGGGGTCGG | Start: 3802 |
| Tm | 60.037 | 59.966 | End: 3979 |
| Size | 20 | 20 | 178 |
| Primer 4 details |
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| Sequence | CCCTTCCAGCAATCGTTCCT | TTTATTCCCGTGTGCTCGCT | Start: 1907 |
| Tm | 60.035 | 60.037 | End: 2166 |
| Size | 20 | 20 | 260 |
| Primer 5 details |
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| Sequence | ACCTCGTGTTTGTGGCAAGA | ATGACCTTCTTGCAGGAGCC | Start: 2971 |
| Tm | 60.107 | 60.035 | End: 3154 |
| Size | 20 | 20 | 184 |
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