ssr_primer
Primer Details for TRINITY_DN75157_c2_g4_i1 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCACTGGGTAAGAGAAGGGC | GGGACGTTCCTCCCCTTATC | Start: 275 |
| Tm | 60.393 | 59.242 | End: 500 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
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| Sequence | ACGGAAAGTGAAGGCATGCA | TTGAAGGTCGCCTTATGCCT | Start: 342 |
| Tm | 60.537 | 59.382 | End: 587 |
| Size | 20 | 20 | 246 |
| Primer 3 details |
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| Sequence | ATGGTGCTTCAGGATCTCCG | GGGACGTTCCTCCCCTTATC | Start: 306 |
| Tm | 59.533 | 59.242 | End: 500 |
| Size | 20 | 20 | 195 |
| Primer 4 details |
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| Sequence | ACGGAAAGTGAAGGCATGCA | GTTGAAGGTCGCCTTATGCC | Start: 342 |
| Tm | 60.537 | 59.267 | End: 588 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
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| Sequence | ACGGAAAGTGAAGGCATGCA | GGGACGTTCCTCCCCTTATC | Start: 342 |
| Tm | 60.537 | 59.242 | End: 500 |
| Size | 20 | 20 | 159 |
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