ssr_primer
Primer Details for TRINITY_DN75150_c2_g1_i2 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTTCTTGGACTCACCGCC | GCCTGGTCAATAACTGGGCT | Start: 628 |
| Tm | 59.965 | 60.034 | End: 842 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | GAATGCACCCAGGAGCTCTT | GGCGGTGAGTCCAAGAAGAA | Start: 439 |
| Tm | 60.035 | 59.965 | End: 647 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | TTCTTCTTGGACTCACCGCC | CTGAGCCTGATGTTGAGCCA | Start: 628 |
| Tm | 59.965 | 60.036 | End: 777 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | CTCGGGTCATGGTTCCAACA | AAGAGCTCCTGGGTGCATTC | Start: 296 |
| Tm | 59.964 | 60.035 | End: 458 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | TTCAGCCCATCACCTCCAAC | GGCGGTGAGTCCAAGAAGAA | Start: 380 |
| Tm | 59.962 | 59.965 | End: 647 |
| Size | 20 | 20 | 268 |
(T)27: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTTCTTGGACTCACCGCC | GCCTGGTCAATAACTGGGCT | Start: 628 |
| Tm | 59.965 | 60.034 | End: 842 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | GAATGCACCCAGGAGCTCTT | GGCGGTGAGTCCAAGAAGAA | Start: 439 |
| Tm | 60.035 | 59.965 | End: 647 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | TTCTTCTTGGACTCACCGCC | CTGAGCCTGATGTTGAGCCA | Start: 628 |
| Tm | 59.965 | 60.036 | End: 777 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | CTCGGGTCATGGTTCCAACA | AAGAGCTCCTGGGTGCATTC | Start: 296 |
| Tm | 59.964 | 60.035 | End: 458 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | TTCAGCCCATCACCTCCAAC | GGCGGTGAGTCCAAGAAGAA | Start: 380 |
| Tm | 59.962 | 59.965 | End: 647 |
| Size | 20 | 20 | 268 |
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