ssr_primer
Primer Details for TRINITY_DN75148_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTAACATGGACCGGCAAGCT | GAGGTTGTGCACACGAAACC | Start: 3803 |
| Tm | 59.962 | 59.971 | End: 3930 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | GGTTTCGTGTGCACAACCTC | TGCACATCTCGTACCCACAC | Start: 3911 |
| Tm | 59.971 | 60.038 | End: 4016 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
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| Sequence | TCGACCGCAAATCCTTCACA | TCCCCCAGGTCTACTAGCAC | Start: 1938 |
| Tm | 59.966 | 60.032 | End: 2163 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
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| Sequence | TCGACCGCAAATCCTTCACA | GTCCCCCAGGTCTACTAGCA | Start: 1938 |
| Tm | 59.966 | 60.032 | End: 2164 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
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| Sequence | CTGGAATGGGCGGTAACACT | TCGAAGTCCCCGGATTACCT | Start: 3048 |
| Tm | 60.035 | 60.033 | End: 3267 |
| Size | 20 | 20 | 220 |
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