Primer Details for TRINITY_DN75119_c5_g3_i1 | |||
|---|---|---|---|
| (T)11cttatgtgtg(T)10gtgggtagttttttttcttgtg(T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTTTTCTTGTGTGTGATGTTGT | GAGAGACGGACAGGCACTTG | Start: 4 |
| Tm | 58.222 | 60.389 | End: 209 |
| Size | 24 | 20 | 206 |
| Primer 2 details | |||
| Sequence | TGTTTTTCTTGTGTGTGATGTTGT | GGACAGAAGGACCCCGAAAA | Start: 4 |
| Tm | 58.222 | 59.601 | End: 151 |
| Size | 24 | 20 | 148 |
| Primer 3 details | |||
| Sequence | TGTTTTTCTTGTGTGTGATGTTGT | GCACTACTGGAGAGACGGAC | Start: 4 |
| Tm | 58.222 | 59.547 | End: 218 |
| Size | 24 | 20 | 215 |
| Primer 4 details | |||
| Sequence | TGTTTTTCTTGTGTGTGATGTTGT | TACTGGAGAGACGGACAGGC | Start: 4 |
| Tm | 58.222 | 60.68 | End: 214 |
| Size | 24 | 20 | 211 |
| Primer 5 details | |||
| Sequence | TGTTTTTCTTGTGTGTGATGTTGTT | GAGAGACGGACAGGCACTTG | Start: 4 |
| Tm | 58.781 | 60.389 | End: 209 |
| Size | 25 | 20 | 206 |
